It has proven to be more objective, time saving, and efficient than all earlier versions.MULTI-DIMENSIONAL MUSCULOSKELETAL RESEARCHīIOQUANT Life Science helps to usher in the next phase of musculoskeletal research with support for XYZ and multi-channel fluorescent image data from optically cleared, intact bone tissue. By combining STEREO with Scion Image, the slide-reading process is simplified and can be performed automatically. As a result, the user can store data on a disk in the format of the STEREO data files. An interface program, BuildFi.exe, was developed to receive data generated from Scion Image and to align sequential tissue plots from up to four serial sections stained with different markers.
Macros for quantitative stereology used in Scion Image were written to customize and simplify the measurement and to generate data needed for building each of the data files. This software integration involves two portions: the building macros and the interface. In order to increase the speed and efficiency of the analytical process, we have integrated the STEREO program with a public domain software, Scion Image.
The data file itself was built by measuring tissue and focal transections through a slide-reading process that involved the manual use of a digitizer. We have previously reported the development of a program, STEREO, which compiles data from carcinogenesis experiments, recording information from tissue sections for the estimation of the number of altered hepatic foci (AHF) per liver and the volume fraction of AHF in liver on a three-dimensional basis. Quantitative stereologic analysis requires considerable data collection and calculation and is thus practical only through the use of computer hardware and software. The resulting quantitative information is useful in both experimental studies and whole-animal investigations for regulatory and safety purposes.
Two-dimensional data obtained from a histological cross-section of a tissue can be utilized to obtain three-dimensional information by the methods of quantitative stereology.
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